Anxiety is an exaggerated feeling of apprehension, uncertainty, and fear. It is an unpleasant state of tension with an anticipation of imminent danger. Benzodiazepines are still the most frequently used drugs for the treatment of generalized anxiety disorder despite their undesirable side effects such as muscle relaxation, sedation, physical dependence, memory disturbance, and interaction with other drugs. The present study was undertaken to evaluate the Standardization of Leaves, anxiolytic activity of Methanolic & Aqueous extract of Tribulus terrestris Linn. The anxiolytic activity was evaluated by Elevated plus Maze & Light Dark Model behavior models. The efficacy of the extract (200 mg/kg) was compared with the standard anxiolytic drugs Diazepam (2 mg/kg). The result showed that the extract significantly increased the number of entries and time spent in the open arm in the elevated plus maze. Both extracts showed significant anxiolytic activity. However Methanolic extract possessed more significant activity as compare to the aqueous extract in Elevated Plus Maze model and Light Dark model.